Mouse Lipoxin B4, LXB4 ELISA Kit

The Mouse Lipoxin B4 (LXB4) ELISA Kit is a high-sensitivity, quantitative immunoassay designed to detect and measure endogenous levels of LXB4 in mouse serum, plasma, cell culture supernatants, and tissue homogenates. Utilizing a competitive ELISA format, the assay employs a pre-coated 96-well microplate with a high-affinity anti-LXB4 capture antibody and a biotinylated detection antibody, enabling specific interaction with Lipoxin B4.

LXB4, a bioactive lipid mediator derived from arachidonic acid via the lipoxygenase pathway, plays a critical role in inflammation resolution, immune modulation, and tissue repair. This ELISA kit provides a dynamic range typically between 10 pg/mL and 1000 pg/mL, with intra-assay and inter-assay coefficients of variation below 10% and 12%, respectively. The assay exhibits minimal cross-reactivity with related eicosanoids, ensuring analytical specificity.