I have done a biotin-streptavidin affinity pull down of my peptides which was pre-equlibrated in buffer with Triton-x-100 which was washed extensively before addition of peptides. There was a lot of Triton and PEG in the samples. The Triton I managed to get rid of using thermo made detergent removal columns. However, I am still stuck with the PEG.
is there a way to get rid of the PEG from my peptides so i can use them for ESI?